BI-O.K. Self-Contained EO Biological Indicator vials are designed for monitoring EO sterilization cycles. The vial contains a crushable ampoule that releases spores into nutrient media, and after 48-hour incubation at 37°C, a color change indicates sterilization efficacy. Yellow media indicates a failed test. Each vial is labeled with a process indicator and space for load info. The product complies with ISO 11138 standards and offers a 24-month shelf life, ensuring traceable and dependable results.